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p rltk  (Promega)

 
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    Structured Review

    Promega p rltk
    P Rltk, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+rltk/prl+tk/pmc10218549-207-22-34
    Average 90 stars, based on 1 article reviews
    p rltk - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Hepatocyte Nuclear Factor 4α (HNF4α) Plays a Controlling Role in Expression of the Retinoic Acid Receptor β ( RARβ ) Gene in Hepatocytes
    Article Snippet: .. RA was purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA) and prepared as stocks at 1 and 10 mM in ethanol. p GL3-Basic-luc, p RLTK, and p GEMT-Easy plasmid vectors were purchased from Promega (Madison, WI, USA) and p cDNA3.1 plasmid vector used for expression was from Invitrogen (Carlsbad, CA, USA). .. MMLV Reverse transcriptase, RNase inhibitor, oligo dT nucleotide, dNTP, and T4 polynucleotide kinase were from Promega (Madison, WI, USA), while High Fidelity Tag DNA polymerase, Lipofectamine 2000 transfection reagent, and Trizol reagent were all from Invitrogen.

    Article Title: Hepatocyte Nuclear Factor 4α (HNF4α) in Coordination With Retinoic Acid Receptors Increases all-trans -Retinoic Acid-Dependent CYP26A1 Gene Expression in HepG2 Human Hepatocytes
    Article Snippet: Rabbit polyclonal IgG antibodies against RARα, RARβ, RXRα, Pol II (H-224), and mouse monoclonal antibody against RARγ, mouse monoclonal IgG2a and goat polyclonal IgG antibodies against HNF4α, and a goat polyclonal IgG goat anti-mouse IgG2a-HRP were all from Santa Cruz Biotechnology (Santa Cruz, CA). .. SuperSignal ® West Pico Reagent containing an enhanced chemoluminescent substrate for detection of horseradish peroxidase as well as HNF4α siRNA together with non-targeting siRNA and DharmaFECT Transfection Reagents were from Thermo Scientific (Rockford, IL). p GL3-Basic- luc , p RLTK, and p GEMT-Easy plasmid vectors were purchased from Promega (Madison, WI) and p cDNA3.1 plasmid vector for expression from Invitrogen (Carsbad, CA). .. Mouse RARα, β, and γ and RXRα clones, each in p SG5 expression plasmid vector, were provided by Dr. Pierre Chambon (Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP/Collège de France, Illkirch-Cedex, Strasbourg, France).

    Article Title: CYP26A1 gene promoter is a useful tool for reporting RAR-mediated retinoid activity
    Article Snippet: .. Plasmid vectors including p GEMT-Easy, p GL3-Basic-luc and p RLTK, were purchased from Promega (Madison, WI) and p cDNA3.1 plasmid vector for protein expression was from Invitrogen (Carsbad, CA). .. MMLV Reverse transcriptase, oligo dT nucleotide, T4 polynucleotide kinase, and T4 DNA ligase were all from Promega, High Fidelity Taq DNA polymerase, Lipofectamine 2000 transfection reagent and Trizol reagent were from Invitrogen.

    Expressing:

    Article Title: Hepatocyte Nuclear Factor 4α (HNF4α) Plays a Controlling Role in Expression of the Retinoic Acid Receptor β ( RARβ ) Gene in Hepatocytes
    Article Snippet: .. RA was purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA) and prepared as stocks at 1 and 10 mM in ethanol. p GL3-Basic-luc, p RLTK, and p GEMT-Easy plasmid vectors were purchased from Promega (Madison, WI, USA) and p cDNA3.1 plasmid vector used for expression was from Invitrogen (Carlsbad, CA, USA). .. MMLV Reverse transcriptase, RNase inhibitor, oligo dT nucleotide, dNTP, and T4 polynucleotide kinase were from Promega (Madison, WI, USA), while High Fidelity Tag DNA polymerase, Lipofectamine 2000 transfection reagent, and Trizol reagent were all from Invitrogen.

    Article Title: Hepatocyte Nuclear Factor 4α (HNF4α) in Coordination With Retinoic Acid Receptors Increases all-trans -Retinoic Acid-Dependent CYP26A1 Gene Expression in HepG2 Human Hepatocytes
    Article Snippet: Rabbit polyclonal IgG antibodies against RARα, RARβ, RXRα, Pol II (H-224), and mouse monoclonal antibody against RARγ, mouse monoclonal IgG2a and goat polyclonal IgG antibodies against HNF4α, and a goat polyclonal IgG goat anti-mouse IgG2a-HRP were all from Santa Cruz Biotechnology (Santa Cruz, CA). .. SuperSignal ® West Pico Reagent containing an enhanced chemoluminescent substrate for detection of horseradish peroxidase as well as HNF4α siRNA together with non-targeting siRNA and DharmaFECT Transfection Reagents were from Thermo Scientific (Rockford, IL). p GL3-Basic- luc , p RLTK, and p GEMT-Easy plasmid vectors were purchased from Promega (Madison, WI) and p cDNA3.1 plasmid vector for expression from Invitrogen (Carsbad, CA). .. Mouse RARα, β, and γ and RXRα clones, each in p SG5 expression plasmid vector, were provided by Dr. Pierre Chambon (Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP/Collège de France, Illkirch-Cedex, Strasbourg, France).

    Article Title: CYP26A1 gene promoter is a useful tool for reporting RAR-mediated retinoid activity
    Article Snippet: .. Plasmid vectors including p GEMT-Easy, p GL3-Basic-luc and p RLTK, were purchased from Promega (Madison, WI) and p cDNA3.1 plasmid vector for protein expression was from Invitrogen (Carsbad, CA). .. MMLV Reverse transcriptase, oligo dT nucleotide, T4 polynucleotide kinase, and T4 DNA ligase were all from Promega, High Fidelity Taq DNA polymerase, Lipofectamine 2000 transfection reagent and Trizol reagent were from Invitrogen.

    Transfection:

    Article Title: Hepatocyte Nuclear Factor 4α (HNF4α) in Coordination With Retinoic Acid Receptors Increases all-trans -Retinoic Acid-Dependent CYP26A1 Gene Expression in HepG2 Human Hepatocytes
    Article Snippet: Rabbit polyclonal IgG antibodies against RARα, RARβ, RXRα, Pol II (H-224), and mouse monoclonal antibody against RARγ, mouse monoclonal IgG2a and goat polyclonal IgG antibodies against HNF4α, and a goat polyclonal IgG goat anti-mouse IgG2a-HRP were all from Santa Cruz Biotechnology (Santa Cruz, CA). .. SuperSignal ® West Pico Reagent containing an enhanced chemoluminescent substrate for detection of horseradish peroxidase as well as HNF4α siRNA together with non-targeting siRNA and DharmaFECT Transfection Reagents were from Thermo Scientific (Rockford, IL). p GL3-Basic- luc , p RLTK, and p GEMT-Easy plasmid vectors were purchased from Promega (Madison, WI) and p cDNA3.1 plasmid vector for expression from Invitrogen (Carsbad, CA). .. Mouse RARα, β, and γ and RXRα clones, each in p SG5 expression plasmid vector, were provided by Dr. Pierre Chambon (Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP/Collège de France, Illkirch-Cedex, Strasbourg, France).



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